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Astaxanthin binding to solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.cbpb.2006.04.011
AuteurRechercher : 1; Rechercher : 1; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
Sujetastaxanthin-binding; Atlantic halibut; Atlantic salmon; fluorescence intensity; haddock; muscle proteins
Résumé
A study was conducted to compare astaxanthin binding ability of solubilized muscle proteins of Atlantic salmon (Salmo salar L.), haddock (Melanogrammus aeglefinus L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Muscle proteins of juvenile Atlantic salmon, haddock and halibut were solubilized by sequential extraction of muscle tissue using low ionic strength solutions. Electrophoretic protein profiles of the six solubilized fractions from these species were similar. Each solubilized fraction from the three species was examined for its relative astaxanthin binding capacity. The amount of bound astaxanthin was significantly different (P < 0.05) among the six fractions of each species. Significant differences in astaxanthin binding were only found for fractions A and E among the species. The amount of bound astaxanthin in various fractions of each species showed a good correlation (R2 = 0.80–0.92) with the ANS (8-anilino-1-naphthalenesulfonate) fluorescence intensity of those fractions. The pattern and extent of astaxanthin binding to the muscle proteins of juvenile salmon, haddock and halibut is comparable to that reported previously for adult Atlantic salmon [Saha, M.R., Ross, N.W., Gill, T.A., Olsen, R.E., Lall, S.P., 2005. Development of a method to assess binding of astaxanthin to Atlantic salmon S. salar L. muscle proteins. Aquacult. Res. 36, 336–343.]. These combined observations suggest that the carotenoid binding capacity of the muscle proteins of salmon is not the limiting factor in the deposition of carotenoid in their flesh.
Date de publication
Conditions d’utilisation
  • Crown Copyright© 2006 Published by Elsevier Inc. All rights reserved.
Dans
  • Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 144, nº 4 (4 mai 2006) : 488–495.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRC1617
NRCC 55556
Numéro NPARC3538239
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Identificateur de l’enregistrementef198444-b6e0-4483-9d50-6105ea75423b
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-22
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