Auteur | Rechercher : Malic, Lidija1; Rechercher : Clime, Liviu1; Rechercher : Daoud, Jamal1; Rechercher : Elmanzalawy, Abdelrahman1; Rechercher : Lukic, Ljuboje1; Rechercher : Lee, Huailo; Rechercher : Tsai, Yuh-Tyng; Rechercher : Jiang, Pei-Shin; Rechercher : Veres, Teodor1 |
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Affiliation | - Conseil national de recherches du Canada. Dispositifs médicaux
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Format | Texte, Article |
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Conférence | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020, October 4-9, 2020, Virtual Online |
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Sujet | biochemical stimulation; cell culture; centrifugal microfluidics; cells; cntrifugation; chemical activation; humidity control; microfluidics |
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Résumé | Complete automation of precisely controlled cell culture microenvironment is herein presented on a centrifugal microfluidic platform with pneumatic active control interface (Powerblade) [1]. The proposed approach to cell culture and stimulation allows for humidity, CO2 and temperature control, analogous to traditional cell culture environments, whilst perfusing cells with the appropriate reagent buffers on-demand. The disposable passive cartridges were fabricated in standard thermoplastic polymers, which allows future use of scalable manufacturing methods suitable for industrial translation. Culture and stimulation of peripheral blood mononuclear cells (PBMCs) is demonstrated and cell activation is validated using ELISA. |
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Date de publication | 2020-10-04 |
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Maison d’édition | Chemical and Biological Microsystems Society |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | fddc3617-de71-4609-b4f8-b9e91bcd72a4 |
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Enregistrement créé | 2022-07-22 |
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Enregistrement modifié | 2022-07-22 |
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